Coding

Part:BBa_K3102004:Design

Designed by: Chloe DOIZELET   Group: iGEM19_Ionis_Paris   (2019-10-03)


RibC (coding for GTP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 359
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 112
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11406


References

Eberhardt S.M.R. et al., "Cloning, sequencing, mapping and hyperexpression of the ribC gene coding for riboflavin synthase of Escherichia coli.", Eur. J. Biochem. 242:712-719(1996)

Lin Z, et al., "Metabolic engineering of Escherichia coli for the production of riboflavin". Microb Cell Fact. 2014;13(1):1–12.

Zhenquan Lin et al., “Metabolic engineering of Escherichia coli for the production of riboflavin," Microbial Cell Factories, 2014, 13:104.